فراوانی ژن‌های پلاسمیدی qnrA، qnrB و qnrS و تعیین حساسیت آنتی بیوتیکی در ایزوله‌های اشرشیاکلی مقاوم به کینولون و فلوروکینولون جدا شده از نمونه‌های بالینی کرمان

Background: Plasmid-mediated quinolone resistance, which complicates treatment, has been increasingly identified in Escherichia coli isolates worldwide. The purpose of this study was to investigate the frequency of plasmid-mediated qnrA, qnrB, and qnrS genes among quinolones resistant E.coli isolated from Kerman hospitals, Iran. Methods: In the current study, 80 E. coli isolates were collected from urine, blood, and wound from Kerman hospitals. Then antibiotic susceptibility test was carried out by using disc diffusion method. Detection of qnrA, qnrB, and qnrS genes were carried out by polymerase chain reaction. Data were evaluated through SPSS v.22. Results: Effective antibiotic against E. coli isolates was imipenem (93.7%) but 58 isolates (72.5%) were resistance to ampicillin which more than others antibiotics. 33 (41.2%) and 15 (18.7%) of 80 Escherichia coli isolates were nalidixic acid and ciprofloxacin-resistant, respectively. Detection of qnr genes demonstrated that 5 (6.2%) isolates were observed qnrS and one (1.2%) were identified qnrA genes isolates. No qnrA gene was identified in our study. Conclusion: Because of different antibiotic resistance patterns in various geographical regions, antimicrobial treatment should be based on local experience. Therefore, prescribing correct antibiotics can prevent the extension of antibiotic resistance through qnr borne bacteria in the future.